Segmentation of 2d-gel Electrophoresis Images
نویسندگان
چکیده
Introduction Two-Dimensional Gel Electrophoresis technique is a convenient and well-established method to separate thousands of proteins on polyacrylamide gels, according to the differences in their net charge and their molecular mass [1]. Its digital output is an image which depicts proteins as bright or dark spots over a noisy and inhomogeneous background. Each protein is characterized by its vertical and horizontal position on the image, as well as its brightness, size and shape. The process of analyzing 2D-gel images includes the following three main stages [2]: spot detection, spot segmentation and spot quantification. The available software packages [2] for 2D-gel image analysis deal with the problem with a different degree of success and the best solution is far from being reach. In this paper, an original approach for the detection and segmentation of spots on 2D-gel images is presented.
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